一般描述
IsoStrip小鼠单克隆抗体分型试剂盒是一个简单的三步法试剂盒,可用于快速鉴定小鼠单克隆抗体。
内容物
内容物
- 显影管,含有预先包被抗小鼠Ig抗体的冻干乳胶珠
- 分型试纸条,预先包被亚类和轻链特异性抗小鼠Ig抗体
特异性
与乳胶珠结合的抗小鼠抗体将与任意同种型的小鼠单克隆抗体反应。试纸条上固定的山羊抗小鼠抗体,能与各种常见的小鼠抗体同种型(IgG1、IgG2a、IgG2b、IgG3、IgM和IgA)以及轻链发生特异性结合。
浓度为1.0 μg/ml时,对所有特异性抗体(IgML除外)的特异性:≥ 2
IgM Lambda, 浓度为1.0 μg/ml时:≥ 1
浓度为1.0 μg/ml时,对所有特异性抗体(IgML除外)的特异性:≥ 2
IgM Lambda, 浓度为1.0 μg/ml时:≥ 1
应用
该试剂盒可用于快速鉴定小鼠单克隆抗体(免疫球蛋白)的分类、亚类和轻链类型。
特点和优势
- 可靠:产生与标准ELISA测定相当的结果
- 快速:从开始到结束仅需5分钟
- 轻松:仅需3个步骤
- 经济:无需任何设备或其他试剂
- 高度特异性:与FBS无交叉反应
包装
1个试剂盒包含2种组分。
产品规格
测定时间:5分钟
样品材料:杂交瘤上清液,纯化小鼠单克隆抗体,腹水
样品材料:杂交瘤上清液,纯化小鼠单克隆抗体,腹水
制备说明
工作溶液:稀释样品
- 从罐中取出所需数量的分型试纸条。取下相同数量的显影管的盖子。
可以用铅笔或实验室记号笔标记显影管,方便识别。 - 使用1% BSA/磷酸盐缓冲盐水(PBS)稀释含有小鼠单克隆抗体的样品,pH 7.2至7.6。
培养上清液样品应以1:10至1:100稀释。腹水样品应按1:20,000稀释。这些是推荐稀释度,具体取决于样品中的抗体浓度。根据我们的经验,稀释样品的单克隆抗体
浓度为0.1至1 g/ml时可获得最佳结果。将150ml上述稀释样品加入显影管中。
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
IsoStrip is a trademark of Roche
仅试剂盒组分
产品编号
说明
- Development Tubes, containing lyophilized latex beads, precoated with anti-mouse-Ig antibodies
- Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies
WGK
nwg
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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