packaging
pkg of 50 discs (82 mm diameter)
manufacturer/tradename
Roche
disc diam.
82 mm
storage temp.
20-25°C
Quality Level
General description
Nylon membrane is a circular, uncharged, microporous, amphoteric, ultra-pure nylon 66 membrane on a polyester support. It has a durable structure with consistent transfer rate.
Surface Properties: Pore size of 1.2 μm. Surface contains 50% amino and 50% carboxyl groups.
Surface Properties: Pore size of 1.2 μm. Surface contains 50% amino and 50% carboxyl groups.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Application
- The membrane discs are specifically suited for:Nonradioactive screening of phage or cosmid libraries with digoxigenin-labeled probes followed by detection with highly sensitive chemiluminescent or chromogenic substrates
- Hybridization with biotinylated or radioactive probes
- It has been used in the colony immunoblotting technique.
K P Karey et al.
Analytical biochemistry, 178(2), 255-259 (1989-05-01)
Western blotting of low molecular weight (Mr) acidic and basic isoelectric point (pI) proteins was studied to optimize detection sensitivities. Radioiodinated epidermal growth factor (EGF, Mr 6045, pI 4.4), transforming growth factor type alpha (TGF alpha, Mr 5623, pI 6.8)
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