usage
sufficient for 1,000 tests (ELISA), sufficient for 600 tests (SEAP)
packaging
pkg of 150 mL
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.
The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).
Features and Benefits
- Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
- High dynamic measuring range: Linear range of more than five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics
Preparation Note
Working solution: Preparation of working solution
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
Other Notes
1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer
2. Enhancer, Emerald II
3. Assay Buffer
For life science research only. Not for use in diagnostic procedures.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash