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Merck
CN

11796895001

Roche

DIG Easy Hyb杂交颗粒

greener alternative

powder, suitable for hybridization

别名:

DIG系统

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About This Item

UNSPSC代码:
41105326

Agency

suitable for

质量水平

形式

powder

用途

sufficient for 6 x 100 mL

制造商/商品名称

Roche

储存条件

(Keep container tightly closed in a dry and well-ventilated place.)

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

hybridization: suitable

颜色

white

pH值(酸碱度)

7,0

溶解性

water: soluble

适用性

suitable for nucleic acid hybridization

环保替代产品分类

储存温度

20-25°C

一般描述

DIG Easy Hyb 是一种杂交溶液,用于与地高辛标记探针一起用于核算印记。DIG Easy Hyb不含甲酰胺,但与含有50%甲酰胺的缓冲液相比,它可以降低杂交温度。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。  DIG系统是灵敏且具有成本效益的方案,可替代放射性标记和放射法检测核酸。有许多已发表论文证明了DIG系统的通用性,因此,使用放射性标记不再是标记用于杂交的DNA的唯一选择。

应用

DIG Easy Hyb杂交颗粒可用于所有的采用非放射性(例如,地高辛标记)核酸探针的膜杂交应用。它专为DIG试剂盒设计。

特点和优势

  • DIG Easy Hyb 是一种随时可用的杂交缓冲液,省时省力。
  • DIG Easy Hyb 为杂交缓冲液提供了更环保的替代品,符合绿色化学的原则。

制备说明

工作溶液:向塑料瓶中小心加入64ml灭菌双蒸水,在37℃下立即搅拌5分钟。提供的6 x 100 ml毫升规格让您可以根据需要轻松“配制”新鲜的DIG Easy Hyb——不需要长期储备溶液。
注:对于RNA印迹应用,DIG Easy Hyb颗粒必须在无RNase的条件下溶解。
无毒性颗粒,塑料瓶分装。复溶为100ml 1x溶液,即用型。

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

DIG Easy Hyb is a trademark of Roche

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

does not flash

闪点(°C)

does not flash


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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访问文档库

Ru-Bing Duan et al.
The Journal of biological chemistry, 289(23), 16006-16015 (2014-04-24)
There are multiple isoforms of p90 ribosomal S6 kinase (RSK), which regulate diverse cellular functions such as cell growth, proliferation, maturation, and motility. However, the relationship between the structures and functions of RSK isoforms remains undetermined. Artemia is a useful
Yanchang Luo et al.
Plant biotechnology (Tokyo, Japan), 37(1), 69-75 (2020-05-05)
Retrotransposons are mobile genetic elements capable of transposition via reverse transcription of RNA intermediates. Rice cultivar Nipponbare contains two nearly identical genomic copies of Tos17, an endogenous copia-like LTR retrotransposon, on chromosomes 7 (Tos17 Chr.7) and 10 (Tos17 Chr.10), respectively.
Chih-Chiang Chan et al.
Current biology : CB, 21(20), 1704-1715 (2011-10-18)
Neurons require highly specialized intracellular membrane trafficking, especially at synapses. Rab GTPases are considered master regulators of membrane trafficking in all cells, and only very few Rabs have known neuron-specific functions. Here, we present the first systematic characterization of neuronal
An Li et al.
Fungal genetics and biology : FG & B, 48(6), 602-611 (2011-02-18)
Several Aspergillus species are well-known for the production of a variety of organic acids. In this study, a cloned based transcriptomics approach was used to identify genes crucial in the biosynthesis pathway for one of these acids, itaconic acid. From
Ziye Rong et al.
Translational vision science & technology, 9(9), 47-47 (2020-09-17)
Fuchs' endothelial corneal dystrophy (FECD) is the leading indication for corneal transplantation. Seventy percent of cases are caused by an intronic CTG triplet repeat expansion in the TCF4 gene that results in accumulation of pathogenic expanded CUG repeat RNA (CUGexp)

实验方案

DIG Easy Hyb™ Granules Protocol & Troubleshooting

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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