产品名称
Annexin-V-FLUOS, solution, suitable for flow cytometry
form
solution
usage
sufficient for 250 tests
packaging
pkg of 500 μL
manufacturer/tradename
Roche
technique(s)
flow cytometry: suitable
shipped in
dry ice
storage temp.
−20°C
Application
细胞凋亡早期，细胞表面发生改变。其中一种质膜改变是磷脂酰丝氨酸 (PS) 从质膜内部向外层的移位，而PS 由此暴露在细胞外表面。
膜联蛋白-V-FLUOS 通过流式细胞术（FACS） 或荧光镜检法，通过结合凋亡细胞膜外叶上的磷脂酰丝氨酸，检测悬浮细胞中的凋亡细胞。 需要用 碘化丙啶 (PI)（举例来说）进行同时标记，以区分凋亡细胞和坏死细胞。
也可以进行其他二级标记，例如 ，用藻红蛋白或 TRITC 标记的单克隆抗体进行膜表面染色，以进一步确定细胞特征。
膜联蛋白-V-FLUOS 通过流式细胞术（FACS） 或荧光镜检法，通过结合凋亡细胞膜外叶上的磷脂酰丝氨酸，检测悬浮细胞中的凋亡细胞。 需要用 碘化丙啶 (PI)（举例来说）进行同时标记，以区分凋亡细胞和坏死细胞。
也可以进行其他二级标记，例如 ，用藻红蛋白或 TRITC 标记的单克隆抗体进行膜表面染色，以进一步确定细胞特征。
Biochem/physiol Actions
膜联蛋白-V 以 Ca 2 + 依赖性方式结合带负电荷的磷脂表面，并显示对磷脂酰丝氨酸的高度亲和力。
General description
膜联蛋白-V-FLUOS 是一种磷脂结合蛋白，对磷脂酰丝氨酸 (PS) 具有高度亲和力。因此，带膜联蛋白-V 细胞表面PS的检测可作为凋亡细胞的标记。可通过流式细胞术或荧光镜检法进行分析。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
工作浓度： 建议使用的 rGFP 量：
检测： 膜联蛋白-V-FLUOS 可在 FACS 分析和免疫化学过程中直接检测，无需二次检测系统。
样品材料：细胞系和新鲜分离的细胞。
- SDS-PAGE（考马斯亮蓝染色）：1μg
- 免疫印迹法：5 ng
检测： 膜联蛋白-V-FLUOS 可在 FACS 分析和免疫化学过程中直接检测，无需二次检测系统。
样品材料：细胞系和新鲜分离的细胞。
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
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商品
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
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