Analysis Note
纯化 5 pg MelJu 细胞 mRNA 后，加入 5 mL 新鲜正常人血制备的裂解物中，用 RNA/DNA 稳定试剂稳定血液/骨髓后，通过 RT-PCR 检测酪氨酸酶 mRNA 的回收率。用血液/骨髓 mRNA 分离试剂盒从稳定裂解物中纯化 mRNA。
注 ：5 pg 的 MelJu 细胞 mRNA 相当于约 5 个黑色素瘤细胞。
Application
易于使用的试剂通过确保核酸酶的完全和即刻失活，增加了核酸检测的准确性。采集时，将 1份血液样本或骨髓穿刺液加入到 10 份试剂中，因此所需的试剂计算量达到最低。
General description
Other Notes
Preparation Note
裂解物可立即使用，在 + 2 至 + 8°C 下储存一天，或在-15 至-25°C下储存长达 1 年。500 mL 足以稳定总共 50 mL 全血或骨髓抽吸物。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
No data available
flash_point_c
No data available
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A poly(A)-mRNA purification procedure applying Boehringer Mannheim's new RNA/ DNA Stabilization Reagent for Blood/Bone Marrow and the new mRNA Isolation Kit for Blood/Bone Marrow, was tested to prove its applicability for the detection of disseminated tumor cells from peripheral blood, by use of the poly(A)-mRNA fraction in combination with a highly sensitive RT-PCR procedure. In order to establish the sensitivity of the technique, peripheral blood samples from healthy donors were spiked with defined numbers of melanoma cells from an established tumor cell line.
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