biological source
Pseudomonas fluorescens
recombinant
expressed in E. coli
form
suspension
specific activity
~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
mol wt
64,000 Da
packaging
pkg of 1 mL (10104973001 [1 mg]), pkg of 1 mL (10104981001 [5 mg])
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white
optimum pH
8.4 (0.1 M in Tris buffer)
pH range
4.6-9.2
solubility
water: soluble
suitability
suitable for enzyme test
UniProt accession no.
foreign activity
ADH <0.01%, LDH <0.5%, NADH oxidase <0.1%, galactosidase <0.01%
storage temp.
2-8°C
General description
β-Galactose Dehydrogenase plays a crucial role in galactose metabolism by catalyzing the pyridine-nucleotide-dependent dehydrogenation of β-D-galactopyranose to yield NADH and D-galactono-l,5-1actone. D-galactose dehydrogenase from P. fluorescens is a member of the oxidoreductases family. It is composed of two identical subunits and has a molecular weight of 64,000. Additionally, the enzyme contains two binding sites.
Application
β-Galactose Dehydrogenase has been used as a reporter enzyme in neuraminidase (NA) kinetic parameters for the natural substrates 6′-Sialyl-N-acetyllactosamine (6SLN) and 3′-Sialyl-N-acetyllactosamine (3SLN).
Biochem/physiol Actions
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Physical form
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6
Analysis Note
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
Other Notes
For life science research only. Not for use in diagnostic procedures.
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
此项目有
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[29] d-Galactose dehydrogenase from Pseudomonas fluorescens
E. Maier and G. Kurz
Methods in Enzymology, 89.0, 176-181 (1982)
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