选择规格
关于此项目
biological source
enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
sterility
non-sterile
form
lyophilized
collagenase activity
>0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)
specific activity
>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
packaging
pkg of 100 mg (10269638001), pkg of 500 mg (11097113001)
manufacturer/tradename
Roche
concentration
1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
technique(s)
tissue processing: suitable
color
light yellow to brown
optimum pH
7.0-8.0
solubility
water: soluble
UniProt accession no.
application(s)
life science and biopharma
sample preparation
foreign activity
proteolytic activities <0.1 U/mg (hemoglobin as the substrate)
storage temp.
2-8°C
General description
Biochem/physiol Actions
Preparation Note
激活剂:Ca2+
工作浓度:约为1 mg/mL(胶原酶:0.1 U/mL,中性蛋白酶:0.8 U/mL
酶活性:
胶原酶:0.1 Wünsch U/mg冻干粉(+ 25 °C,4-苯基-偶氮苄基-氧基羰基-Pro-Leu-Gly-Pro-D-Arg作为底物)
中性蛋白酶:0.8 Wünsch U/mg冻干粉(+ 37 °C,酪蛋白为底物,pH 7.5)
污染蛋白水解活性:<0.1 U/mg 冻干粉 (血红蛋白作为底物)
工作溶液:工作溶液制备 将酶溶于水中,使其终浓度为100mg/mL。用PBS(不含Ca2+/Mg2+)稀释至终浓度为1 mg/mL,并采用无菌0.2 μm 过滤膜过滤。
无菌溶液的酶浓度:胶原酶,0.1 U/mL PBS; 中性蛋白酶®, 0.8 U/mL PBS。
抑制剂:
胶原酶抑制剂:EDTA, EGTA
血清不能抑制胶原酶/中性蛋白酶。
保存条件(工作溶液):-15 至 -25 °C
复溶的储备溶液在-15至-25 °C下稳定。
注:适当分装保存并避免反复冻融!
复溶于水中(100 mg/mL)后续稀释采用PBS(磷酸缓冲盐)(不含Ca2+/Mg2+)。
干燥储存
Other Notes
Legal Information
signalword
Danger
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash

