biological source
yeast (Candida boidini)
recombinant
expressed in E. coli
form
lyophilized
specific activity
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
mol wt
74 kDa
packaging
pkg of 250 U (10837016001), pkg of 80 U (10244678001)
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
off-white
optimum pH
7.5-8.5
solubility
water: soluble 10 g/L
suitability
suitable for gel electrophoresis
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.0500%, LDH <0.0500%, MDH <0.1000%
storage temp.
2-8°C
General description
Biochem/physiol Actions
底物特异性和Km:
甲酸脱氢酶仅与甲酸盐(在30℃和pH 7.5条件下,Km = 13 mM;在25℃和pH 7.5条件下,11mM)和NAD(在30℃和pH 7.5条件下,Km 0.09 mM)反应。它不与乙酸盐、草酸盐、乳酸盐、琥珀酸盐、丙酸盐或抗坏血酸盐反应,也不会还原NADP。
甲酸脱氢酶仅与甲酸盐(在30℃和pH 7.5条件下,Km = 13 mM;在25℃和pH 7.5条件下,11mM)和NAD(在30℃和pH 7.5条件下,Km 0.09 mM)反应。它不与乙酸盐、草酸盐、乳酸盐、琥珀酸盐、丙酸盐或抗坏血酸盐反应,也不会还原NADP。
Preparation Note
干燥储存
Analysis Note
污染物:<0.005%的LDH和ADH,<0.1%的MDH
Other Notes
一单位(U)甲酸脱氢酶在+25 °C、pH 7.6下将1 μmol甲酸氧化成CO2。上述测定中,每氧化1 mmol甲酸将产生1mmol NADH。
仅用于生命科学研究。不可用于诊断。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash