产品名称
L-苹果酸脱氢酶 (L-MDH), from pig heart (mitochondrial)
biological source
Porcine heart (mitochondrial)
form
solution
suspension
specific activity
~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
packaging
pkg of 1 mL (10127248001 solution)
pkg of 1 mL (10127256001 suspension)
pkg of 5 mL (10127914001suspension)
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white
optimum pH
7.4-7.5(reduction of oxaloacetate)
9.2-9.5(oxidation of malate)
solubility
water: miscible
suitability
suitable for Western blot
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
Fumarase <0.01%
GIDH 0.001%
GOT 0.001%
GPT 0.00185%
LDH <0.01%
NADH oxidase <0.001%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
Porcine ... MDH2(397039)
Analysis Note
污染物： <0.002% GOT、<0.01% 延胡索酸酶和 LDH，各 luM，<0.02%HK、<0.002%PGI
Application
来自猪心脏的L-苹果酸脱氢酶已用于测量PEPCK（磷酸烯醇丙酮酸羧化激酶）活性。PEPCK生产的草酰乙酸可通过NADH氧化而还原为苹果酸，然后使用分光光度计在340 nm处进行测定。该酶还用于通过在340 nm处光谱测定NADH的还原来测量草酰乙酸。
Biochem/physiol Actions
猪心脏中的L-苹果酸脱氢酶将L-苹果酸氧化催化为草酰乙酸。该酶是一种在三羧酸循环中起作用的NAD依赖性线粒体脱氢酶。它是苹果酸-天冬氨酸转运蛋白的组成部分，以苹果酸形式跨内线粒体膜运输还原当量。
General description
L-苹果酸：NAD + 氧化还原酶
L-苹果酸脱氢酶是一种核基因产物，由 24 个氨基酸末端的信号肽合成。这种肽在酶转运至线粒体基质的过程中被蛋白水解裂解。
L-苹果酸脱氢酶是一种核基因产物，由 24 个氨基酸末端的信号肽合成。这种肽在酶转运至线粒体基质的过程中被蛋白水解裂解。
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
50% 甘油溶液 (v/v)，pH 值约为7
悬浮于3.2 M硫酸铵溶液中，pH约为6
Preparation Note
活化剂：–磷酸盐
-砷酸盐
– Mg2+
– Zn2+
-砷酸盐
– Mg2+
– Zn2+
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
低风险生物材料
动植物源性产品
此项目有
Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum).
Osorio, S et al.
Bio-protocol, 4 (2014)
Agata M Pudlik et al.
Journal of bacteriology, 193(3), 706-714 (2010-12-01)
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated
S L Roderick et al.
The Journal of biological chemistry, 261(20), 9461-9464 (1986-07-15)
In a previous study, we reported the apparent similarity between a low resolution electron density map of mitochondrial malate dehydrogenase and a model of cytoplasmic malate dehydrogenase (Roderick, S. L., and Banaszak, L. J. (1983) J. Biol. Chem. 258, 11636-11642).
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