09-0615
溴化乙锭
≥95.0%
别名:
3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵
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关于此项目
经验公式（希尔记法）:
C21H20BrN3
化学文摘社编号:
分子量:
394.31
EC Number:
214-984-6
UNSPSC Code:
12161505
PubChem Substance ID:
Beilstein/REAXYS Number:
3642536
MDL number:
InChI key
ZMMJGEGLRURXTF-UHFFFAOYSA-N
InChI
1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
SMILES string
[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
assay
≥95.0%
availability
available only in Japan
mp
260-262 °C (dec.) (lit.)
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Application
溴化乙锭 (EtBr) 是 PAGE 或琼脂糖凝胶电泳最常用的核酸染色剂。EtBr 的荧光在与双链 RNA 结合后可增加 21 倍，在与双链 DNA 结合后增加 25 倍，因此在低染色浓度（10μg/ml）下无需本底脱色。溴化乙锭已用于大量核酸荧光测定。 它已被证明可与单链 DNA（虽然没有那么强）和三链 DNA 结合。由于它能与 DNA 结合，EtBr 是 DNA 聚合酶的抑制剂。
插入双链 DNA 和 RNA 的移码诱变剂。
插入双链 DNA 和 RNA 的移码诱变剂。
for biological purposes
Biochem/physiol Actions
溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。
Preparation Note
对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
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