09-0617
溴化乙啶 溶液
10 mg/mL
别名:
3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵
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关于此项目
经验公式（希尔记法）:
C21H20BrN3
化学文摘社编号:
分子量:
394.31
PubChem Substance ID:
UNSPSC Code:
12161505
Beilstein/REAXYS Number:
3642536
MDL number:
SMILES string
[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
InChI key
ZMMJGEGLRURXTF-UHFFFAOYSA-N
form
liquid
availability
available only in Japan
concentration
10 mg/mL
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Application
溴化乙啶 (EtBr) 为聚丙烯酰胺凝胶电泳或琼脂糖凝胶电泳中最常用的核酸染料。EtBr 在结合到双链 RNA 上时其荧光可提高 21 倍，在结合到双链 DNA 上可提高 25 倍，从而使得在低染料浓度 (10μg/ml) 下无需背景脱色。溴化乙啶已用于核酸的许多荧光分析。已表明它可结合到单链 DNA（虽然强度不高）和三链 DNA 上。由于 EtBr 能够结合到 DNA 上，因此它是 DNA 聚合酶的抑制剂。
for biological purposes
Biochem/physiol Actions
溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。
Preparation Note
对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Muta. 2
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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