Merck
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文件

15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

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别名:
Nylon 66-Blotting membranes, type B

物料

Nylon

质量水平

100

等级

for molecular biology

形式

roll

表面覆盖度

surface (quaternary ammonium groups) ≥99%

孔径

0.45 μm pore size

储存温度

room temp

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相关类别

一般描述

Positively charged nylon cast on polyester-support; membrane size 0,2m x 3m (rolled up)

应用

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and
Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

实验方案

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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