16658
5-溴-4-氯-3-吲哚基-N-乙酰-β-D-氨基半乳糖苷
reagent for selection of recombinant bacterial clones
别名:
5-溴-4-氯-3-吲哚基-N-乙酰氨基-2-脱氧-β-D-吡喃半乳糖苷, X-GalNAc
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关于此项目
经验公式（希尔记法）:
C16H18BrClN2O6
化学文摘社编号:
分子量:
449.68
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
产品名称
5-溴-4-氯-3-吲哚基-N-乙酰-β-D-氨基半乳糖苷, reagent for selection of recombinant bacterial clones
InChI
1S/C16H18BrClN2O6/c1-6(22)20-13-15(24)14(23)10(5-21)26-16(13)25-9-4-19-8-3-2-7(17)12(18)11(8)9/h2-4,10,13-16,19,21,23-24H,5H2,1H3,(H,20,22)/t10-,13-,14+,15-,16-/m1/s1
SMILES string
CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI key
SUWPNTKTZYIFQT-LMXXTMHSSA-N
grade
Molecular Biology
sterility
non-sterile
assay
≥98.0% (TLC)
form
powder
mp
≥220 °C (lit.)
solubility
DMF: soluble
DMSO: soluble
suitability
suitable for β-galactosidase test
storage temp.
−20°C
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Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for the detection of Candida albicans.
Biochem/physiol Actions
When X-GalNAc is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GalNAc turn blue in color, allowing for easy differentiation between lac+ and lac- colonies.
General description
5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide (X-GalNAc)is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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