Quality Segment
form
liquid
technique(s)
thin layer chromatography (TLC): suitable
impurities
1-butanol, acetic acid
density
0.823 g/mL at 20 °C
General description
Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.
Application
1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid
Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
存储类别
3 - Flammable liquids
wgk
WGK 2
flash_point_f
93.2 °F - closed cup
flash_point_c
34 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter