form
powder, powder with small lumps
specific activity
≥150 U/mg protein (protein ca. 40%)
mol wt
107 kDa by gel filtration, 57 kDa by SDS-PAGE
shipped in
wet ice
storage temp.
−20°C
InChI
1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
InChI key
DHUYKLYJBKXDBM-UHFFFAOYSA-N
General description
牛肝脏中的蛋白质二硫键异构酶 (PDI) 是一种分子量为 107 kDa 的同源二聚体(凝胶过滤),已报告单体的分子量为 57 kDa (SDS-PAGE)。该酶是总碳水化合物含量为 12% 的糖蛋白,由 4.6% 的甘露糖、2.5% 的半乳糖、1.4% 的 NANA 和 3.5% 的 2-乙酰胺基-2-脱氧葡萄糖组成。
Other Notes
1 unit corresponds to the amount of enzyme, which, in a glutathione-dithiothreitol-system, causes a change of absorbance of 0.001 per minute at pH 4.0 and 25°C.
Enzyme that accelerates the exchange reaction between disulfide bridges in proteins