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经验公式(希尔记法):
C27H27Br2NaO5S
化学文摘社编号:
分子量:
646.36
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
产品名称
溴百里酚蓝 钠盐 溶液, 0.04 wt. % in H2O
InChI
1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
SMILES string
[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
InChI key
NMKFVGALBGZKGW-FKWCIMQXSA-M
form
liquid
concentration
0.04 wt. % in H2O
pH
6.0-7.6, yellow to blue
density
0.99 g/mL at 25 °C (lit.)
λmax
615 nm
ε (extinction coefficient)
≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Application
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Biochem/physiol Actions
Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.
General description
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
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