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关于此项目
经验公式(希尔记法):
C27H27Br2NaO5S
化学文摘社编号:
分子量:
646.36
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
form
liquid
Quality Segment
concentration
0.04 wt. % in H2O
pH
6.0-7.6, yellow to blue
density
0.99 g/mL at 25 °C (lit.)
λmax
615 nm
ε (extinction coefficient)
≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L, ≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
InChI
1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
InChI key
NMKFVGALBGZKGW-FKWCIMQXSA-M
General description
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.
Application
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Biochem/physiol Actions
Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.
存储类别
10 - Combustible liquids
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
PPE(个人防护设备)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)