生物来源
Porcine
质量水平
表单
powder
质量
ultrahigh gel strength
保质期
limited shelf life, expiry date on the label
缺失
9.5-12.5% loss on drying
透射比
450 nm, ≥85%
620 nm, ≥95%
pH值(酸碱度)
5.20-5.60
电导率
120-190 μS/cm at 30 °C (1%)
粘度
5.10-5.80 mPa.s
凝胶强度
280-302 g BloomAOAC
溶解性
H2O: 67 mg/mL at 60 °C
痕量阳离子
Ca: ≤100 mg/kg
应用
microbiology
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应用
This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.
Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.
制备说明
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG
其他说明
明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。
免责声明
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
储存分类代码
11 - Combustible Solids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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