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Merck
CN

39465

明胶 来源于猪皮肤

suitable for microbiology, ultrahigh gel strength

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化学文摘社编号:
UNSPSC Code:
41106212
EC Number:
232-554-6
NACRES:
NA.85
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biological source

Porcine

form

powder

quality

ultrahigh gel strength

shelf life

limited shelf life, expiry date on the label

loss

9.5-12.5% loss on drying

transmittance

450 nm, ≥85%, 620 nm, ≥95%

pH

5.20-5.60

conductivity

120-190 μS/cm at 30 °C (1%)

viscosity

5.10-5.80 mPa.s

gel strength

280-302 g BloomAOAC

solubility

H2O: 67 mg/mL at 60 °C

cation traces

Ca: ≤100 mg/kg

application(s)

microbiology

Quality Level

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Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

Other Notes

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

Disclaimer

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

存储类别

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Lot/Batch Number

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Monica A Serban et al.
Methods in molecular biology (Clifton, N.J.), 1001, 133-143 (2013-03-16)
Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and
Takeo Furuya et al.
The journal of spinal cord medicine, 36(2), 134-139 (2013-07-03)
Besides stimulating angiogenesis or cell survival, basic fibroblast growth factor (bFGF) has the potential for protecting neurons in the injured spinal cord. To investigate the effects of a sustained-release system of bFGF from gelatin hydrogel (GH) in a rat spinal
Panuphong Pootawang et al.
Journal of nanoscience and nanotechnology, 13(1), 589-592 (2013-05-08)
The environmental concern pays much attention to the recent cause of the global warming effect. The reduction of the chemical uses is one of many ways to avoid this crucial problem. Herein, the green process for silver nanometallic particle formation
Simon Young et al.
Journal of controlled release : official journal of the Controlled Release Society, 109(1-3), 256-274 (2005-11-04)
Gelatin is a commonly used natural polymer which is derived from collagen. The isoelectric point of gelatin can be modified during the fabrication process to yield either a negatively charged acidic gelatin, or a positively charged basic gelatin at physiological
Kimberly E Hawkins et al.
Molecular brain, 6, 14-14 (2013-03-26)
Matrix metalloproteinases are important factors in the molecular mechanisms leading to neuronal injury in many neurological disorders. Matrix metalloproteinase (MMP)-9 is up-regulated after cerebral ischemia and neuroinflammation and is actively involved in blood-brain barrier disruption. Current methods of measuring MMP-9

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