Merck
CN

48900

Sigma-Aldrich

Giemsa 染料,改良溶液

(for the staining (of cellular blood components and blood parasites))

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别名:
Giemsa 染液 溶液, 天青曙红亚甲蓝
CAS号:
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

类型

(for research use only)

质量水平

保质期

limited shelf life

技术

microbe id | staining: suitable

适用性

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

SMILES字符串

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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应用

根据G.Clark(ed.),Staining Procedures,4th edition,Williams&Wilkins,173(1981)测试染色血涂片。根据Pappenheim的研究,将May-Grünwald溶液用于巴氏染色。

注意

储存于20°C

原理

当 Giemsa 染液对血涂片进行染色时,白细胞的细胞核和细胞质将呈现特征性的蓝色或粉红色。使用经纯化的伊红和噻嗪染料可使批间差异最小化。

外形

溶于甘油/甲醇5:24(v/v)中的天青B/天青II-伊红/亚甲蓝1:12:2(w/w/w)溶液;染料总含量:0.6%(w/w)

重悬

在甲醇中固定10分钟;空气干燥;在Giemasa溶液中沉浸45分钟;用蒸馏水进行冲洗;空气干燥

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

靶器官

Eyes

储存分类代码

3 - Flammable liquids

WGK

WGK 2

闪点(°F)

53.6 °F

闪点(°C)

12 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品

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