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Nitrate Reagent Disks Kit is used to detect an organism’s ability to reduce nitrate. The test involves the detection of the enzyme nitrate reductase, which causes the reduction of nitrate in the presence of a suitable electron donor to nitrite, which can be tested by an appropriate colorimetric reagent. Almost all Enterobacteriaceae reduce nitrate. The nitrate in the disc is broken down to nitrite when nitrate reductase positive culture is grown in presence of these discs. The reduction of nitrate (NO₃) to nitrite (NO₂) and subsequently to nitrogen gas (N₂) usually takes place under anaerobic conditions, in which an organism derives its oxygen from nitrate. Most facultative anaerobes can reduce nitrate in the absence of oxygen.

Nitrate Reagent Disks Kit is used for rapid detection and confirmation of an organism’s ability to reduce nitrate. They find their application in various sectors such as the food and dairy industry, water industry, pharmaceutical laboratory testing, cosmetic industry, environmental and sanitary testing, clinical diagnostic, etc.

Sterile filter paper discs (50pcs, diameter 6mm) impregnated with Nitrate reagent (Cat. No. 08086).
Rehydrating fluids (Cat. No. 43505, 5ml)