跳转至内容
Merck
CN

51558

HEPES 缓冲盐溶液

BioUltra, Molecular Biology, 2× concentrate

别名:

HEPES缓冲液

登录 查看组织和合同定价。

选择尺寸


关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

HEPES 缓冲盐溶液, BioUltra, Molecular Biology, 2× concentrate

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

Quality Level

正在寻找类似产品? 访问 产品对比指南

Application

HEPES 缓冲盐水已被使用:
  • 用于制备质粒 DNA
  • 作为制备用于 DNA 构建载体的转染主混合物的试剂
  • 开发用于细胞渗漏测定的水凝胶前体

General description

HEPES 缓冲盐水是组织培养缓冲液,其用于维持细胞培养物的 pH。它是水溶性的,不依赖大气。HEPES 在氯化钠溶液中破坏镁。

Preparation Note

浓度(H2O):10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l 葡萄糖

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Immunology, 10, 10-10 (2001)
A new approach to neural cell culture for long-term studies
Potter SM and DeMarse TB
Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
C P Leamon et al.
Bioconjugate chemistry, 10(6), 947-957 (1999-11-24)
Poly(ethylene glycol) of various sizes was used as a molecular spacer to separate the cell-targeting ligand, folate, from the surface of poly-L-lysine. The resulting ternary macromolecule (pLys-PEG-folate) was investigated in various formulations for its ability to transfect reporter plasmids into
Jong-Yuh Cherng et al.
Journal of materials science. Materials in medicine, 21(5), 1543-1551 (2010-02-06)
To improve the cytotoxicity of PEI25k and the transfection efficiency of poly(beta-amino ester) with DNA, we synthesized a poly(beta-amino ester), PEDP, bearing ester linkages in the backbone and tertiary amines in the backbone and side chain and prepared a binary
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B11 (1989)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持