grade
Molecular Biology
Quality Level
product line
BioUltra
form
liquid
quality
2× concentrate
composition
dextrose, 2.0 g/L , HEPES, 10 g/L , KCl, 0.74 g/L , NaCl, 16 g/L , Na2HPO4.2H2O, 0.27 g/L
concentration
2 ×
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
refractive index
n20/D 1.339
pH
7.2 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.06, λ: 280 nm Amax: 0.04
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
HEPES 缓冲盐水是组织培养缓冲液，其用于维持细胞培养物的 pH。它是水溶性的，不依赖大气。HEPES 在氯化钠溶液中破坏镁。
Application
HEPES 缓冲盐水已被使用：
- 用于制备质粒 DNA
- 作为制备用于 DNA 构建载体的转染主混合物的试剂
- 开发用于细胞渗漏测定的水凝胶前体
Preparation Note
浓度（H2O）：10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l 葡萄糖
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Immunology, 10, 10-10 (2001)
A new approach to neural cell culture for long-term studies
Potter SM and DeMarse TB
Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
Labeling embryonic mouse central nervous system cells by in utero electroporation
Tabata H and Nakajima K
Development, Growth & Differentiation, 50(6), 507-511 (2008)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A-046-1ML | 04061833326114 |
| PHR3846-100MG | 04065268836538 |
| Y0000845 | 04061833805312 |
| Y0000878 | 04061833796658 |
| A0132-1G | 04061833326909 |
| A0132-5G | 04061833326923 |
| A0132-100G | 04061832088488 |
| A0132-25G | 04061833326916 |
| 51558-50ML | 04061832497006 |
| 1091960025 | 04022536937946 |