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Merck
CN

60983

Kovac 吲哚试剂

suitable for microbiology

别名:

对二甲氨基苯甲醛 溶液, 按照 Kovac 方法的吲哚试剂

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关于此项目

经验公式（希尔记法）:
C9H11NO
化学文摘社编号:
100-10-7
分子量:
149.19
Beilstein:
4132845
MDL编号:
MFCD00003381
UNSPSC代码:
41171621
PubChem化学物质编号:
329759335
NACRES:
NA.85

主要文件

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保质期

limited shelf life, expiry date on the label

质量水平

100

组成

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

技术

microbe id | specific enzyme detection: suitable

折射率

n20/D 1.400-1.420

应用

agriculture
clinical testing
environmental
food and beverages
microbiology

储存温度

2-8°C

适用性

Escherichia coli
coliforms

SMILES字符串

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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相关类别

一般描述

Kovac′s吲哚试剂是一种含有4-（二甲胺基）苯甲醛和盐酸的正丁醇溶液。

应用

Kovac′s吲哚试剂用于计数存储、解冻和熟肉饼等碎牛肉样本的大肠杆菌O157:H7。它还用于计数脱水黑水中的大肠杆菌。

生化/生理作用

Kovac′s吲哚试剂用于检测吲哚并帮助鉴定吲哚阳性和吲哚阴性微生物。在色氨酸酶存在的情况下，大肠杆菌可以将色氨酸分解为吲哚和α丙氨酸。试剂中的p-氨基苯甲醛与吲哚发生反应，生成溶于酒精、乙醚和氯仿的樱桃红络合物。建议使用不含葡萄糖的培养基和色氨酸含量高的蛋白胨进行测试。

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Central nervous system, Respiratory system

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

96.8 °F

闪点(°C)

36 °C

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品
易制毒化学品（3类）
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN3398
BCCL0925
BCCK8015
BCCM2245
BCCJ6939

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Stavros G Manios et al.
Meat science, 101, 25-32 (2014-12-03)
The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After
Ragnhildur Gunnarsdóttir et al.
Environmental science & technology, 46(22), 12408-12416 (2012-11-02)
Wastewater treatment in many Arctic regions is inadequate, even nonexisting. Natural freezing of wastewater in those areas may be beneficial for reduction of microorganisms. The aim of this study was to investigate the effect of long-term freezing, and repeated freezing
G L Lombard et al.
Journal of clinical microbiology, 18(3), 609-613 (1983-09-01)
Three reagents for detecting indole, Kovac, Ehrlich, and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the three reagents, gave a positive reaction with 445 of 449
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
Spectrophotometric determination of certain cephalosporins through oxidation with cerium(IV) and 1-chlorobenzotriazole.
M M Ayad et al.
Journal of pharmaceutical and biomedical analysis, 20(3), 557-564 (2000-03-04)
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