sterility
sterile; 0.45 μm filtered
form
dry powder
shelf life
limited shelf life, expiry date on the label
technique(s)
microbiological culture: suitable
solubility
sterile water: 100 mg/mL
application(s)
food and beverages, microbiology
shipped in
ambient
storage temp.
2-8°C
suitability
selective for bacteria: Yersinia enterocolitica
Quality Level
General description
Y. enterocolitica Millichrome™ plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vials sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
Application
Y. enterocolitica Millichrome™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.
Preparation Note
- Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
- Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
- Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
- Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
- Swirl gently to homogenize.
- Pour into sterile Petri dishes.
Analysis Note
The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature
Other Notes
Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
Legal Information
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
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商品
Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
相关内容
68022 Y. enterocolitica Millichrome™ plus Supplement
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