保质期
limited shelf life, expiry date on the label
质量水平
组成
Agar, 15.0 g/L
Bromocresol Green free acid, 0.022 g/L
Calcium carbonate, 5.0 g/L
Calcium pantothenate, 2.0 g/L
Citric acid, 1.10 g/L
Dextrose, 10.0 g/L
Dipotassium phosphate, 0.5 g/L
Ferrous sulfate, 0.01 g/L
Magnesium sulfate, 0.2 g/L
Manganese sulfate, 0.01 g/L
Monopotassium phosphate, 0.5 g/L
Peptonized Milk, 20.0 g/L
Sodium chloride, 0.01 g/L
Tomato Juice Broth, 20.0 g/L
Tween® 80, 0.5 g/L
Yeast extract, 10.0 g/L
最终pH
5.5±0.2 (25 °C)
应用
microbiology
应用
LMDA (Lee′s Multi-Differential Agar) is a differential solid medium for the detection and enumeration of a variety of brewery bacteria and yeast.
制备说明
Mix 84.8 grams of the medium in 1 liter of purified water until evenly dispersed. Heat with repeated stirring until boiling to fully dissolve. Autoclave at 121°C for 15 minutes. Cool medium to approx. 45-50°C in a water bath. Gently and thoroughly swirl the medium, without agitation, in order to uniformly suspend the calcium carbonate precipitate. Dispense the medium into sterile petri dishes.
法律信息
TWEEN is a registered trademark of Croda International PLC
储存分类代码
11 - Combustible Solids
WGK
WGK 3
法规信息
新产品
此项目有
实验方案
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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