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Merck
CN

88597

Millipore

过氧化氢 溶液

3%, suitable for microbiology

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别名:
过氧化氢酶试验, 过氧化氢酶试验,H2O2
经验公式(希尔记法):
H2O2
CAS号:
分子量:
34.01
Beilstein:
3587191
MDL编号:
UNSPSC代码:
41171621
PubChem化学物质编号:
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

质量水平

蒸汽压

23.3 mmHg ( 30 °C)

检测方案

2.8-3.2% (redox titration)

形式

solution

保质期

limited shelf life, expiry date on the label

储存条件

protect from light

浓度

1-5%

技术

microbe id | specific enzyme detection: suitable

颜色

colorless

pH值(酸碱度)

6-8 (25 °C)

mp

0.0 °C

密度

1.000 g/cm3

应用

clinical testing
environmental
food and beverages
veterinary

microbiology

适用性

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES字符串

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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一般描述

过氧化氢可分解为水和氧气,常用于微生物学研究。过氧化氢酶可催化这一分解反应,许多好氧菌和兼性厌氧菌中都含有这种酶。 在细菌培养物中添加少量过氧化氢,可检测培养物中是否存在活性过氧化氢酶。如存在,过氧化氢酶会水解过氧化氢,释放氧气气泡。观察可发现培养物冒泡。没有冒泡代表培养物中不存在过氧化氢酶。过氧化氢酶试验常用于微生物鉴定和鉴别,不同种类的细菌具有不同水平的过氧化氢酶活性。例如,葡萄球菌具有较高的过氧化氢酶活性,链球菌无此活性。过氧化氢酶试验也用于区分好氧菌和兼性厌氧菌,前者会产生氧气作为副产物(具有过氧化氢酶活性),后者利用氧气,但不会产生氧气副产物(具有或不具有过氧化氢酶活性)。

应用

过氧化氢具有强氧化性,在微生物领域中起多种重要作用。例如:
  • 消毒剂:过氧化氢可用作微生物实验室表面、材料和设备的消毒剂。施加表面后,过氧化氢会与细菌、真菌等反应,通过氧化作用破坏这些有机体。
  • 灭菌:过氧化氢常用于微生物灭菌程序,尤其是医药行业。 它可有效杀灭许多其他类型的消毒剂无法杀死的孢子。
  • 微生物检测:过氧化氢可检测细菌中的过氧化氢酶,适合用于微生物的鉴定和鉴别。根据对过氧化氢水解的催化能力,可区分不同种类的细菌。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Faceshields, Gloves, Goggles


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Choi S and Love PE
Bio-protocol, 8(1) (2018)
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Osamu Sakai et al.
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The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control

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Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

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实验方案

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