902020
Desthiobiotin-PEG3-Azide
别名:
(4S,5R)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
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选择尺寸
关于此项目
经验公式（希尔记法）:
C18H34N6O5
化学文摘社编号:
分子量:
414.50
UNSPSC Code:
51171641
NACRES:
NA.22
MDL number:
SMILES string
[N+](=[N-])=NCCOCCOCCOCCNC(=O)CCCCC[C@@H]1NC(=O)N[C@@H]1C
InChI
1S/C18H34N6O5/c1-15-16(23-18(26)22-15)5-3-2-4-6-17(25)20-7-9-27-11-13-29-14-12-28-10-8-21-24-19/h15-16H,2-14H2,1H3,(H,20,25)(H2,22,23,26)/t15-,16+/m1/s1
InChI key
JIQVKRRASYKCEL-CVEARBPZSA-N
form
paste
storage temp.
−20°C
Application
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
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