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The trehalose fermentation test is based on the fermentation ability of trehalose which results in the production of acid as end products, the pH of the medium will drop, and an indicator changes color to red. Some organisms produce additional CO₂ gas which can be made visible with a Durham’s tube. The trehalose discs are aseptically added to any sugar-free basal media to test the ability of the organism to hydrolyze trehalose, a non-reducing disaccharide, as the sole source of carbon and energy during fermentation. Diverse organisms like Enterobacteriaceae, Streptococci, and Enterococci can ferment trehalose.

Trehalose Discs are recommended to detect trehalose fermenting bacteria in food and environmental samples. They find their application in various sectors such as the food and dairy industry, water industry, pharmaceutical laboratory testing, cosmetic industry, environmental and sanitary testing, clinical diagnostic, etc.