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Merck
CN

93283

Sigma-Aldrich

Tris-EDTA 缓冲液

BioUltra, for molecular biology, pH 8.0

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别名:
TE缓冲液, TE 缓冲液 溶液
MDL编号:
UNSPSC代码:
41105319
eCl@ss:
32129211
PubChem化学物质编号:
NACRES:
NA.25

等级

for molecular biology

质量水平

产品线

BioUltra

形式

liquid

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH值(酸碱度)

8.0±0.2 (25 °C)

痕量阳离子

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

紫外吸收

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.01

应用

sample preparation

SMILES字符串

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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一般描述

Tris EDTA是生物学研究中的一种缓冲液。它是Tris碱和EDTA(一种金属螯合剂)的组合。Tris提供缓冲能力,可在生物样本中维持稳定的pH值,而EDTA则通过螯合二价金属离子来防止金属离子的干扰。Tris EDTA常用于分子生物学技术中,如DNA和RNA样本制备、电泳和酶促反应。它通过抑制核酸酶和金属依赖性酶的活性,帮助维持核酸的稳定性和完整性。

应用

TE缓冲液在一般提取、悬浮、洗涤和储存缓冲液以及DNA或RNA的制备中很有用。它通常用于DNA测序、限制性酶消化、连接和聚合酶链式反应(PCR)等应用。

特点和优势

  • BioUltra级别 - 适用于许多分子生物学应用
  • 无DNase、RNase、磷酸酶和蛋白酶
  • 高纯度,无微量金属。

其他说明

如需了解生化试剂系列的更多信息,请填写此表

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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