Quality Segment
product line
BioUltra
form
liquid
quality
10× concentrate
composition
glycine, 1.92 M , TRIS, 0.25 M
impurities
insoluble matter, passes filter test, proteases, none detected
ign. residue
≤0.2%
pH
8.5-8.7
anion traces
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, NH4+: ≤200 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.1
Application
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.
Other Notes
从聚丙烯酰胺凝胶到硝化纤维片的蛋白质电泳转移：步骤和一些应用
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves