DMT-2′O-TC-rA(ib) Phosphoramidite

InChI

1S/C49H61N8O10PS2/c1-32(2)46(58)54-44-41-45(52-30-51-44)56(31-53-41)47-43(66-48(69)55-24-27-70(59,60)28-25-55)42(67-68(64-26-12-23-50)57(33(3)4)34(5)6)40(65-47)29-63-49(35-13-10-9-11-14-35,36-15-19-38(61-7)20-16-36)37-17-21-39(62-8)22-18-37/h9-11,13-22,30

InChI key

JDHHUACDFJOZGR-LCVXBTAASA-N

SMILES string

S=C(N1CCS(CC1)(=O)=O)O[C@@H]2[C@H](OP(N(C(C)C)C(C)C)OCCC#N)[C@H](O[C@H]2N3C4=C(N=C3)C(NC(C(C)C)=O)=NC=N4)COC(C5=CC=C(OC)C=C5)(C6=CC=C(OC)C=C6)C7=CC=CC=C7

assay

≥98% (³¹P-NMR), ≥98.0% (reversed phase HPLC)

technique(s)

oligo synthesis: suitable

impurities

≤-0.5% 31P-NMR P(III) impurites 145-155ppm (³¹P-NMR), ≤-0.5% Impurity 3′-TC Isomer (reversed phase HPLC)

compatibility

configured for ABI

nucleoside profile

base: adenosine
base protecting group: isobutyryl
2' protecting group: TC
5' protecting group: DMT

storage temp.

-10 to -25°C

Quality Level

200