Merck
CN

A8456

Sigma-Aldrich

4-(2-氨乙基)苯磺酰氟 盐酸盐

≥97.0% (HPLC)

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别名:
AEBSF
经验公式(希尔记法):
C8H10FNO2S · HCl
CAS号:
分子量:
239.69
Beilstein:
7604627
MDL编号:
PubChem化学物质编号:
NACRES:
NA.77

生物来源

synthetic (organic)

质量水平

检测方案

≥97.0% (HPLC)

形式

powder

mp

183-191  °C

溶解性

H2O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)

储存温度

−20°C

SMILES字符串

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChI key

WRDABNWSWOHGMS-UHFFFAOYSA-N

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应用

4-(2-氨乙基)苯磺酰氟盐酸盐(AEBSF)已用作:
  • 血浆样品中的蛋白酶抑制剂,以防止酰基化胃饥饿素(AG)降解
  • SVSP抑制或AEBSF测定中的蛇毒丝氨酸蛋白酶(SVSP)抑制剂,以测定SVSP的参与度对纤维蛋白原凝血活性的影响
  • NP-40裂解缓冲液的组分,以重悬细胞微球制备细胞裂解物

建议使用浓度为 0.1 至 1mM。

生化/生理作用

AEBSF可抑制乙酰胆碱酯酶(AChE)。
4-(2-氨乙基)苯磺酰氟盐酸盐(AEBSF)是一种不可逆丝氨酸蛋白酶抑制剂。抑制常数与苯甲磺酰氟(PMSF)和二异丙基氟磷酸(DFP)相似。 经证实,AEBSF可抑制胰蛋白酶、胰凝乳蛋白酶、纤溶酶、激肽释放酶和凝血酶。作为PMSF和DFP的替代品,AEBSF具有较低的毒性,并提高了在水中的溶解性和水溶液中的稳定性。AEBSF可用于浓度高达0.25 mM的细胞培养。

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Corr. 1A

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. In what solvents is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, soluble?

    It is highly soluble in water and in DMSO, at 50 mg/mL (approximately 200 mM), yielding a clear colorless solution. AEBSF may be used in cell culture medium at concentrations up to 0.25 mM.  

  4. How stable is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, in solution?

    Solutions in water are slightly acidic and retain inhibitory activity for up to six months when stored refrigerated. Solutions at pHs above 7 are less stable.  Stock solutions should be stored at a pH less than 7. If a final pH of greater than 7 is required, the pH adjustment should be done shortly before use.

  5. What is the recommended working concentration for Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride?

    Our recommended working concentration range is 0.1 mM to 1 mM; the optimal concentration will depend on the application.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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