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B2261

双苯并咪唑 H 33342 三盐酸盐

≥98% (HPLC)

别名:

2′-(4-乙氧基苯基)-5-(4-甲基-1-哌嗪基)- 2,5′-双-1H-苯并咪唑 三盐酸盐, HOE 33342, Hoechst 33342, 双苯并咪唑

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关于此项目

经验公式（希尔记法）:
C27H28N6O · 3HCl · xH2O
化学文摘社编号:
875756-97-1
分子量:
561.93 (anhydrous basis)
NACRES:
NA.47
PubChem Substance ID:
24891639
UNSPSC Code:
12171500
MDL number:
MFCD00012678
Beilstein/REAXYS Number:
1234011
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Quality Level

300

assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow

pH

1.7 (20 °C)

solubility

H2O: 20 mg/mL, phosphate buffer: precipitates

suitability

suitable for fluorescence

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6

InChI

1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H

InChI key

FYEVKHPLBHLWHK-UHFFFAOYSA-N

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相关类别

General description

双苯甲酰亚胺H 33342的荧光性质：
游离染料：最大激发波长= 340 nm，最大发射波长= 510 nm(5 mM HEPES, 10 mM NaCl, pH 7.0)DNA复合物：最大激发波长= 355 nm，最大发射波长= 465 nm (5 mM HEPES, 10 mM NaCl, pH 7.0)

Application

Bisbenzimide Hoechst 33342是双链高AT含量区域的特异染色剂，经证可替代几种已知的DNA嵌入剂。这种荧光染料已用于根据DNA含量分选活细胞选、流式细胞术DNA含量测定、活细胞染色质分布可视化。它已用于检测细胞BrdU掺入、细胞凋亡和细胞周期分配的初始阶段研究,。正在分裂或复制的染色体不会被这种染料染色。
适用于DNA，染色体和细胞核染色。可用于荧光显微镜或流式细胞术。
最大激发波=346nm
最大发射波长=460nm

Biochem/physiol Actions

膜透性，具有低细胞毒性荧光DNA染料，插入在DNA的A-T区域。

Preparation Note

该产品可溶于水（50 mg/ml），形成澄清溶液。2％溶液的pH为1.9。现已观测发现此物质可从磷酸盐缓冲溶液中沉淀出来。
如果在2-8°C黑暗环境存放，水溶液可稳定保存1个月。

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000523950
0000523950
0000502647
0000502647
0000502663

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P E Mozdziak et al.
Cytometry, 41(2), 89-95 (2000-09-26)
5-Bromo-2'-deoxyuridine (BrdU) is a powerful compound to study the mitotic activity of a cell. Most techniques that identify BrdU-labeled cells require conditions that kill the cells. However, the fluorescence intensity of the membrane-permeable Hoechst dyes is reduced by the incorporation
D J Arndt-Jovin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25(7), 585-589 (1977-07-01)
The methods for measuring the deoxyribonucleic acid content of individual mammalian cells and sorting them on the basis of this parameter have until now required fixation or other treatment which renders the cells nonviable. Using a class of bis-benzimidazole dyes
Basic helix-loop-helix transcriptional factor MyoR regulates BMP-7 in acute kidney injury.
Kamiura N, et al.
American Journal of Physiology: Renal Physiology, 304, F1159-F1166 (2013)
M G Ormerod et al.
Cytometry, 14(6), 595-602 (1993-01-01)
We have recently developed a method for the separation and quantification of viable apoptotic cells without the need for permeabilisation or fixation of the cells. The method is based on the observation that apoptotic rat thymocytes fluoresce more brightly than
The transforming activity of Wnt effectors correlates with their ability to induce the accumulation of mammary progenitor cells.
Liu BY, et al.
Proceedings of the National Academy of Sciences of the USA, 101, 4158-4163 (2004)
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