主要文件

查看所有文档

M0178

Middlebrook 7H9 肉汤基础

Name: Middlebrook 7H9 肉汤基础
Brand: SIAL

NutriSelect^® Plus, powder, for mycobacteria, pkg of 500 g

别名:

Mycobacterial culture media

登录并加购，请在购物车里查看协议价、货期和发货地。

选择规格

变更视图

关于此项目

NACRES:

NA.85

UNSPSC Code:

41171607

Manufacturer/tradename:

NutriSelect^® Plus

Form:

powder

Suitability:

mycobacteria

Application(s):

clinical testing
food and beverages
life science and biopharma
microbiology
veterinary

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

属性

sterility

non-sterile

Quality Segment

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect^® Plus

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

final pH

6.6±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology
veterinary

storage temp.

2-25°C

suitability

mycobacteria

说明

General description

Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species.
Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals.
Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.

Application

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

Preparation Note

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN^® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

成分 (g/L)
硫酸铵，0.50
磷酸二钠，2.50
磷酸二氢钾，1.00
氯化钠，0.10
硫酸镁，0.05
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
柠檬酸铁铵，0.04
L-谷氨酸，0.50
吡哆素，0.001
生物素，0.0005

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

存储类别

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c