产品名称
TRIS醋酸盐-EDTA缓冲液, pH 8.3, pHast Pack™, powder
InChI
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
SMILES string
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI key
HGEVZDLYZYVYHD-UHFFFAOYSA-N
product line
pHast Pack™
form
powder
pH
8.3
suitability
suitable for gel electrophoresis (after dilution to working concentration)
suitable for gel electrophoresis
foreign activity
DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected
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Application
TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液，也可用于非变性RNA琼脂糖凝胶电泳。 双链DNA在TAE中比在其他缓冲液中运行更快，但也可能在延长的电泳过程中耗尽。 在延长的电泳过程中，缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。 浓缩TAE缓冲液稀释后得到1× TAE缓冲液，加入40 mM醋酸三酯和1 mM EDTA，pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
Suitable as a running and gel preparation buffer in:
- DNA agarose gel electrophoresis
- Non-denaturing RNA agarose gel electrophoresis for RNA >1500 bases
- Denaturing gradient gel electrophoresis
- Northern and Southern blotting
Preparation Note
由经过生物技术性能认证的Trizma碱(T6066)和分子生物学试剂EDTA二钠盐(E5134)制备而成。溶液也含有醋酸(A6283)；粉状混合物含Trizma醋酸(T1258)。
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Features and Benefits
- Ready for use – saves time and effort
- No measuring and pH adjusting needed
- Eliminate exposure to toxic chemicals to prepare buffers
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
General description
Tris Acetate-EDTA (TAE) buffer, with a pH range of 8.1 – 8.4, is typically used for agarose gel electrophoresis, especially for the recovery of DNA. For larger double-stranded DNA fragments (>12kB) TAE buffer is preferred. However, the buffering capacity can become exhausted if electrophoresis time is extended. Buffer circulation or replacement during electrophoresis can remedy the lower buffering capacity.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Packaging
Foil pouches
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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