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About This Item

UNSPSC代码:
12161700
NACRES:
NA.25
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质量水平

产品线

pHast Pack

表单

powder

储存条件

(Tightly closed. Dry)

杂质

≤5 ppm Heavy Metals (lead)

颜色

white

pH值(酸碱度)

8.1

痕量阳离子

Fe: ≤10 ppm

异质活性

DNase, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected

储存温度

room temp

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一般描述

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

应用

Intended for use in:
  • Cell culture (sterile filter recommended)
  • Western blot
  • Protein electrophoresis
  • DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

特点和优势

  • Ready-to-use for quick buffer prep
  • No measuring and adjusting pH
  • Saves time and minimizes effort in the lab
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

重悬

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

储存及稳定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 1


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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