产品名称
Tris buffer, Pre-set, pH 8.1, pHast Pack™, powder
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
8.1
cation traces
Fe: ≤10 ppm
foreign activity
DNase, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected
storage temp.
room temp
Quality Level
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Application
Intended for use in:
- Cell culture (sterile filter recommended)
- Western blot
- Protein electrophoresis
- DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers
Features and Benefits
- Ready-to-use for quick buffer prep
- No measuring and adjusting pH
- Saves time and minimizes effort in the lab
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
General description
Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
wgk
WGK 1
法规信息
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