RDD029
异硫氰酸胍
for molecular biology, free-flowing, Redi-Dri™, ≥99.0%
别名:
硫氰酸亚脲, 硫氰酸胍
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线性分子式:
NH2C(=NH)NH2 · HSCN
化学文摘社编号:
分子量:
118.16
NACRES:
NA.28
UNSPSC Code:
12352107
Beilstein/REAXYS Number:
3563461
MDL number:
产品名称
异硫氰酸胍, for molecular biology, free-flowing, Redi-Dri™, ≥99.0%
SMILES string
SC#N.NC(N)=N
InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N
product line
Redi-Dri™
assay
≥99.0%
quality
for molecular biology
free-flowing
mp
120-122 °C (lit.)
foreign activity
DNAse, none detected
RNAse, none detected
Quality Level
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Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Application
离液剂和强效变性剂;溶解细胞。
Guanidine thiocyanate is commonly used for research:
- to denature proteins in cell lysis buffer
- to isolate and purify RNA from cells and tissues
- to extract DNA from cellular and viral genomes
Biochem/physiol Actions
Guanadine thiocyanate is strong protein denaturant commonly combinded with phenol to lyse tissues, cells, and proteins during RNA isolation assay.
Features and Benefits
- Redi-Dri™ makes working with hygroscopic chemicals easier
- Controls moisture and powder remains free-flowing
- Safer to handle and weigh out for routine use in lab
- Eliminates clumps and maintains product moisture and quality attributes
General description
Guanadine thiocyanate (GTC) is a chaotropic agent and strong denaturant that is used in research for protein degredation in vitro. This is useful for DNA or RNA isolation and purification. GTC is a key reagent for inactivation of endogenous DNases and RNase for molecular biology techniques used to isolate nucliec acids from tissues, cells, or viral genomes.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Martin Roszkowski et al.
Frontiers in cell and developmental biology, 9, 648274-648274 (2021-05-11)
The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it
Peter L Roberts et al.
Biologicals : journal of the International Association of Biological Standardization, 35(4), 343-347 (2007-05-23)
Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by
Serazhutdin A Abdullaev et al.
Radiation and environmental biophysics, 54(3), 297-304 (2015-05-04)
Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear
商品
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
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