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安全信息

S2002

Sigma-Aldrich

叠氮化钠

ReagentPlus®, ≥99.5%

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别名:
NSC 3072, 叠氮酸 钠盐
线性分子式:
NaN3
CAS号:
26628-22-8
分子量:
65.01
EC 号:
247-852-1
MDL编号:
MFCD00003536
PubChem化学物质编号:
24899504
NACRES:
NA.21

生物来源

synthetic (inorganic)

质量水平

200

产品线

ReagentPlus®

检测方案

≥99.5%

形式

powder

储存条件

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

技术

cell based assay: suitable
immunohistochemistry: suitable

颜色

white

mp

370-425 °C

溶解性

soluble 65 g/L at 20 °C (completely)

适用性

suitable for chromatography
suitable for derivatization

应用

sample preparation

SMILES字符串

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

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相关类别

一般描述

叠氮化钠 (NaN3) 是一种有效的抑制剂–兼无味的水溶性结晶粉末。该物质公认应用广泛，在分子生物学、细胞培养和生化研究中发挥着关键作用。叠氮化钠通常用作水溶性实验室试剂和生物液中的抑菌防腐剂。它还是一种代谢抑制剂，可抑制氧化磷酸化。在细胞培养应用中，它可通过防止微生物污染保持细胞系的完整性。其应用扩展到分子生物学领域，可帮助保存核酸供下游分析使用，确保结果的保真度。

应用

Baylis-Hillman乙酸酯如2-（乙酰氧基苯基甲基）丙烯酸乙酯可在水相介质中通过叠氮化钠进行亲核取代，以形成(E)-2-叠氮基甲基-3-苯基丙烯酸乙酯。
在组织病理学中，叠氮化钠可用于制备和储存组织样品。 此外，它还可用作整体免疫标记的染色缓冲液（HEPES 缓冲液）的成分。
可作为以下应用的催化剂：

  • 氧化脱羧
  • 迈克尔加成反应（Michael Addition）

合成
  • 蓝色荧光共聚物的试剂
  • 金属膦酸盐
  • 胺化芳烃

生化/生理作用

叠氮化钠在临床上用作红细胞计数稀释液中的防腐剂。在各种科学应用（包括流式细胞分析）中，它可防止荧光表面结合抗体加帽和内化。

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

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警示用语：

Danger

危险分类

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

靶器官

Brain

补充剂危害

EUH032

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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  1. What concentrations of Product S2002, Sodium azide, are used as a preservative?

    Typical concentrations for use are from 0.02 % to 0.1 %.

  2. How can I determine the concentration of sodium azide in solution?

    Sodium azide forms a 3,5-dinitrobenzoyl derivative that can be quantified by liquid chromatography.  This method is reported in the Journal of Chromatography, 5, 597 (1982).

  3. Are there any hazards associated with the disposal of Product S2002, Sodium azide?

    Sodium azide can form metal azides.  If you have metal pipes for your plumbing, you should not dispose of solutions down them. 

  4. How stable are solutions of Product S2002, Sodium azide?

    At a neutral pH sodium azide solutions are fairly stable.  Under acidic conditions it is converted to hydrazoic acid, a volatile gas.

  5. How does Product S2002, Sodium azide, inhibit certain metalloenzymes?

    The mechanism of sodium azide inhibition may be due to metal ion complexation and displacement from the enzyme.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Three-dimensional cellular visualization in mouse apical periodontitis using combined whole-mount staining and optical tissue clearing
Kento Tazawa , Hajime Sasaki
Journal of oral biosciences, 65, 132-135 (2023)
Calcium uptake by rat kidney mitochondria.
DeLuca HF and Engstrom GW.
Proceedings of the National Academy of Sciences of the USA, 47(11), 1744-1750 (1961)
A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
Patricia A Arndt , George Garratty
Transfusion Medicine Reviews, 24(3), 174-192 (2010)
Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
Yadav JS, et al.
Tetrahedron Letters, 46(16), 2761-2763 (2005)
Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
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实验方案

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

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ELISA 操作步骤

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