Merck
CN

S9430

Sigma-Aldrich

SYBR®Green I核酸凝胶染液

greener alternative

10,000 × in DMSO

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别名:
DNA stain, SYBR® green gel dye, safer gel stain
MDL编号:
NACRES:
NA.52

形式

solution

质量水平

用途

 mL sufficient for 100 mini-gels

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

浓度

10,000 × in DMSO

技术

PCR: suitable

环保替代产品分类

储存温度

−20°C

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一般描述

SYBR® Green I是一种特制的不对称花菁染料,用于检测核酸。这种成分由N-烷基化苯并噻唑或苯并噁唑环系统组成,通过单次甲基桥连接吡啶鎓或喹啉鎓环系统。SYBR Green I与dsDNA的小沟结合,激发波长480 nm。其荧光发射峰波长为520nm。

应用

SYBR® Green I核酸凝胶染色剂已用于:
  • dsDNA定量
  • 聚合酶链式反应(PCR),用于DNA染色
  • Comet测定技术
  • 评估精子膜的完整性
  • 扩增DNA 目视检查(环介导等温扩增(LAMP)法)
  • 用作流式细胞术中的荧光染料
  • 实时定量逆转录聚合酶链式反应(qRT-PCR),用于逆转录cDNA染色

特点和优势

  • 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
  • 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA,无需任何预洗涤步骤
  • 在 Ames 试验中,它的致突变性比溴化乙锭小
  • 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
  • 适用于 DNA 数量有限的许多应用
  • SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性,包括 Hind III 和 EcoR I
  • 这种染色剂无需通过凝胶酶解和连接技术去除
  • SYBR Green I 是溴化乙锭染色的绿色替代产品

储存及稳定性

将产品储存在–20 °C。稀释后的染色溶液避光可在2–8 °C保存数周或室温保存3-4天。

法律信息

SYBR is a registered trademark of Life Technologies

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

201.2 °F - closed cup

闪点(°C)

94 °C - closed cup

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?  

    Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine.  A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine.  This will allow for better staining. 

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

Whole transcriptome amplification (WTA) is a method often used to amplify small quantities of reverse transcribed RNA, or degraded RNA for direct use in Next-Generation Sequencing (NGS) workflows. Discover our SeqPlex™-I WTA library preparation kit for producing libraries that are ready for direct input onto Illumina® NGS flow cells.

实验方案

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

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