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Merck
CN

W1761

Wilkins Chalgren 厌氧琼脂

suitable for microbiology, NutriSelect® Plus

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NACRES:
NA.85
UNSPSC Code:
41171605
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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

cleaning products
clinical testing
cosmetics
food and beverages
personal care, microbiology

suitability

anaerobic bacteria, selective for Bacteroides spp., selective for Bifidobacterium spp., selective for Clostridium spp.

Quality Level

Application

用于分离厌氧菌。

Preparation Note

Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates.For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement (Cat. No. N8781) or 2 vial of G.N. Spore Anaerobic Supplement (Cat. No. G3789) as desired into the sterile molten medium before pouring into sterile petri plates.

Other Notes

成分 (g/L)
酶水解酪蛋白,10.00
动物组织消化物,10.00
酵母提取物,5.00
右旋糖,1.00
氯化钠,5.00
L-精氨酸,1.00
丙酮酸钠,1.00
血晶素,0.005
甲萘醌,0.0005
琼脂,10.00
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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T D Wilkins et al.
Antimicrobial agents and chemotherapy, 10(6), 926-928 (1976-12-01)
A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%)

商品

Bifidobacteria-selective media detects beneficial bacteria, aiding quality control in microbiology labs.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

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