biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 00010402-DNA-5UG, pkg of vial of cells 00010402-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0
Reactivity
Anti-Factor H, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
Quality Level
Immunogen
Human Factor H
Biochem/physiol Actions
Mouse x Mouse hybridoma
The hybridoma line OX-23 was raised by immunizing BALB/c mice with Factor H and fusion of spleen cells with NS0 mouse myeloma cells. The antibody was shown to detect human complement control protein, Factor H. OX-23, also known as MRC OX-23, reduced the increase in cofactor activity observed in the presence of OX-24 (Sigma Catalogue no. 00010403). It was reported that OX-23 and OX-24 react with different epitopes on the Factor H molecule.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Daniel Ajona et al.
Cancer research, 64(17), 6310-6318 (2004-09-03)
The complement system is important in immunosurveillance against tumors. However, malignant cells are usually resistant to complement-mediated lysis. In this study, we examine the expression of factor H, an inhibitor of complement activation, and factor H-like protein 1 (FHL-1), its
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