Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto^™ 514 or Alexa Fluor^™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 511 (PBS pH 7.4), 512 (H₂O), 517 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 85 000 (PBS pH 7.4), 92 000 (H₂O), 95 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,24 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 530 (PBS pH 7.4), 530 (H₂O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)

Designed and tested for fluorescent super-resolution microscopy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

abberior is a registered trademark of Abberior GmbH