01632
Atto 520-Biotin
BioReagent, suitable for fluorescence, ≥90% (HPLC)
别名:
Biotin-Atto 520
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关于此项目
经验公式（希尔记法）:
C37H53ClN6O8S
分子量:
777.37
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32
MDL number:
InChI
1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;
InChI key
OCYRRKMPTKPPHX-QITHAKLPSA-N
product line
BioReagent
assay
≥90% (HPLC)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
solubility
DMF: soluble, ethanol: soluble, methanol: soluble
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 522-528 nm Amax
suitability
suitable for fluorescence
storage temp.
2-8°C
Application
Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Siddharth Nanguneri et al.
PloS one, 7(5), e38098-e38098 (2012-06-05)
Three-dimensional fluorescence imaging of thick tissue samples with near-molecular resolution remains a fundamental challenge in the life sciences. To tackle this, we developed tomoSTORM, an approach combining single-molecule localization-based super-resolution microscopy with array tomography of structurally intact brain tissue. Consecutive
Hee-Jin Jeong et al.
Biosensors & bioelectronics, 40(1), 17-23 (2012-07-17)
Protein phosphorylation is a key event in intracellular signal transduction, and fluorescent biosensor for the specific phosphorylation event in a target protein is considered highly useful as a tool of cellular biology and drug screening. Vimentin, the most abundant intermediate
Manoj Kumbhakar et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 10(4), 629-633 (2009-01-30)
Complex dynamics of combined long-range fluorescence resonance energy transfer (FRET) and short-range electron transfer (ET) processes in a single double-stranded DNA (dsDNA) molecule (see figure) reveals that FRET remains almost unaltered in the presence of ET. Present systems also demonstrate
Vladimir Mekler et al.
The Journal of biological chemistry, 286(1), 270-279 (2010-10-19)
Promoter recognition by RNA polymerase is a key point in gene expression and a target of regulation. Bacterial RNA polymerase binds promoters in the form of the holoenzyme, with the σ specificity subunit being primarily responsible for promoter recognition. Free
Christopher A Teske et al.
The journal of physical chemistry. B, 109(28), 13811-13817 (2006-07-21)
Confocal laser scanning microscopy (CLSM) is being increasingly used for observing protein uptake in porous chromatography resins. Recent CLSM studies have revealed the possible existence of a nondiffusive protein transport mechanism. Observing protein uptake with CLSM requires labeling the protein
商品
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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