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01632

Atto 520-Biotin

Name: Atto 520-Biotin
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

别名:

Biotin-Atto 520

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About This Item

经验公式（希尔记法）:

C₃₇H₅₃ClN₆O₈S

分子量:

777.37

MDL编号:

MFCD04040671

UNSPSC代码:

12352108

eCl@ss:

34058011

PubChem化学物质编号:

329747336

NACRES:

NA.32

产品线

BioReagent

质量水平

100

方案

≥90% (HPLC)

表单

powder

制造商/商品名称

ATTO-TEC GmbH

溶解性

DMF: soluble
ethanol: soluble
methanol: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 522-528 nm A_max

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES字符串

[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C
[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C

InChI

1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;

InChI key

OCYRRKMPTKPPHX-QITHAKLPSA-N

应用

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

法律信息

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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