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Merck
CN

01673

吖啶黄

BioReagent, suitable for fluorescence, ≥90% (AT)

别名:

中性吖啶黄素, 优黄素, 氯代3,6-二氨基-10-甲基吖啶和3,6-二氨基吖啶(原黄素)混合物

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关于此项目

经验公式(希尔记法):
C14H14ClN3
化学文摘社编号:
分子量:
259.73
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
MDL number:
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产品名称

吖啶黄, BioReagent, suitable for fluorescence, ≥90% (AT)

SMILES string

[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46
[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46

InChI

1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H

InChI key

PEJLNXHANOHNSU-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (AT)

form

powder

composition

Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC

solubility

H2O: 0.33 g/mL (lit.)(lit.)

fluorescence

λex 463 nm; λem 490 nm in ethanol

suitability

suitable for fluorescence

Quality Level

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Application

吖啶黄用于荧光标记高分子量 RNA 分子 。
各种溶剂中的激发和发射波长 :
  • 甲醇:λex = 424 nm;λem = 518 nm
  • 乙醇:λex = 426 nm;λem = 524 nm
  • 丙醇:λex = 430 nm;λem = 512 nm
  • 丁醇:λex = 430 nm;λem = 526 nm
  • 甲酰胺:λex = 434 nm;λem = 524 nm
  • 甘油:λex = 432 nm;λem = 540 nm
  • 水:λex = 416 nm;λem = 514 nm

不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子(当与罗丹明 6G 作为受体配对时),起到 pH 传感器的作用 。

Other Notes

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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M Stöhr et al.
Stain technology, 53(4), 205-215 (1978-07-01)
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with
Zheqing Cai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17462-17467 (2013-10-09)
Both preclinical and clinical studies suggest that brief cycles of ischemia and reperfusion in the arm or leg may protect the heart against injury following prolonged coronary artery occlusion and reperfusion, a phenomenon known as remote ischemic preconditioning. Recent studies
Shizuka Nakayama et al.
Chemical communications (Cambridge, England), 47(16), 4766-4768 (2011-03-15)
The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP
Jun Fang et al.
Journal of biomolecular structure & dynamics, 31(4), 385-392 (2012-08-11)
The resistance-nodulation-cell division family multidrug transporter Acriflavine resistance protein B (AcrB) from Eschericha coli is an obligate homotrimer. Approximately, 45% of the inter-subunit interface is contributed by a protruding loop (also referred to as the thumb) and its corresponding binding
Saadia Hassan et al.
Cancer science, 102(12), 2206-2213 (2011-09-14)
A high-throughput screen of the cytotoxic activity of 2000 molecules from a commercial library in three human colon cancer cell lines and two normal cell types identified the acridine acriflavin to be a colorectal cancer (CRC) active drug. Acriflavine was

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