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经验公式(希尔记法):
C12H11NO4
化学文摘社编号:
分子量:
233.22
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352106
MDL number:
Beilstein/REAXYS Number:
7927205
产品名称
7-氨基-4-甲基香豆素-3-乙酸, BioReagent, suitable for fluorescence, ~90% (HPLC)
SMILES string
CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12
CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12
InChI key
QEQDLKUMPUDNPG-UHFFFAOYSA-N
InChI
1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
product line
BioReagent
assay
~90% (HPLC)
form
powder
solubility
DMF: soluble
DMSO: soluble
aqueous base: soluble
fluorescence
λex 350 nm; λem 433 nm in methanol
suitability
suitable for fluorescence
Quality Level
Application
AMCA是一种用于标记蛋白的试剂。其在345 nm处具有特征吸收峰以及在440-460 nm处具有荧光发射(蓝色区域);然而,当与蛋白质缀合时,吸收峰移至355 nm,并在440-460 nm处发射荧光。其具有高的荧光产量和足够的斯托克斯位移(100 nm),并且相对光稳定 。其相对容易被激活并偶联至肽的N端氨基,从而形成酸稳定的酰胺键 。
Other Notes
有效的蓝色荧光团,可用于免疫荧光, λabs ~350 nm, λem ~440 nm
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
B Ulfhake et al.
Journal of neuroscience methods, 40(1), 39-48 (1991-11-01)
This paper describes the implementation of an ultraviolet (UV) laser (Spectra Physics 171-18 with 3 lines: 334, 351 and 364 nm in UV) as light source for fluorescence confocal scanning microscopy. With this instrument it is possible to use fluorophores
Melissa J Leyva et al.
Chemistry & biology, 17(11), 1189-1200 (2010-11-26)
Huntington's Disease (HD) is characterized by a mutation in the huntingtin (Htt) gene encoding an expansion of glutamine repeats on the N terminus of the Htt protein. Numerous studies have identified Htt proteolysis as a critical pathological event in HD postmortem
C Otto et al.
The American journal of physiology, 271(1 Pt 1), C210-C217 (1996-07-01)
Dipeptide uptake was studied in primary cultures from rat anterior pituitaries by use of radiolabeled carnosine and the fluorescent dipeptide derivative beta-Ala-Lys-N epsilon-AMCA (AMCA is 7-amino-4-methylcoumarin-3-acetic acid). Fluorescence microscopic studies revealed that the reporter peptide specifically accumulated in the S-100
M W Wessendorf et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 38(1), 87-94 (1990-01-01)
7-Amino-4-methylcoumarin-3-acetic acid (AMCA) has been found to be a useful fluorophore for immunofluorescence. The present study describes a spectrophotometric method for determining the ratio of moles AMCA to moles protein (or the f/p ratio) in an AMCA-conjugated IgG. The concentration
P M Nederlof et al.
Cytometry, 10(1), 20-27 (1989-01-01)
A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. Three chromosome-specific repetitive
商品
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。
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