Shmac 5 cell line

Prostate cancer moderately well differentiated, early stage

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 12,14
D7S820: 7,12
THO1: 6,9.3
TPOX: 8,12
vWA: 15,17

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.