11012001
FLO-1 cell line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
别名:
FLO1 Cells
生物来源
human esophagus
质量水平
包装
tube of 5 μg 11012001-DNA-5UG
pkg of vial of cells 11012001-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
P53 mutation c.830G>T
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
应用
For complete product information, please see ECACC
生化/生理作用
Distal oesophageal adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16
包装
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
制备说明
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
S J Hughes et al.
Cancer research, 57(24), 5571-5578 (1998-01-04)
This study describes Fas (CD95) expression in Barrett's esophagus, adenocarcinomas of the esophagus, and three esophageal adenocarcinoma cell lines. Immunohistochemical analysis of Barrett's esophagus demonstrated cell surface expression of Fas protein. In contrast, 30.5% of esophageal adenocarcinomas examined by immunohistochemical
Mathieu Francois Derouet et al.
PloS one, 9(12), e115589-e115589 (2015-01-01)
Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC) pre
Sarah Derks et al.
Cancer immunology research, 3(10), 1123-1129 (2015-06-18)
Esophageal adenocarcinoma is an increasingly common disease with a dismal 5-year survival rate of 10% to 15%. In the first systematic evaluation of the PD-1 pathway in esophageal adenocarcinoma, we identify expression of PD-L2 in cancer cells in 51.7% of
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the
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