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Merck
CN

12103C

胎牛血清

USA origin, suitable for cell culture

别名:

FBS, FCS, 血清

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

FBS仍然是一种常用的培养基补充剂,因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子,并通过难以在无血清培养基(SFM)系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。

Application

胎牛血清在不同类型的细胞培养物中用作补充剂,例如:
  • 在添加胎牛血清和其它成分的 Dulbecco ′改良 Eagle′培养基中PC12 细胞的生长。
  • HeLa 细胞是在含有胎牛血清和其它成分的罗斯威尔公园纪念研究所 (RPMI) 1640 培养基中供养。
  • BaF3 细胞培养物(细胞在补充胎牛血清和其它成分的 RPMI 1640 培养基中供养)。
  • 原代海马细胞培养物(细胞在补充有热灭活胎牛血清和其它成分的Eagle′最低必需培养基供养)。
  • HeLa、Cos1 和 Cos7 细胞在含有胎牛血清和其它成分的 Dulbecco 改良 Eagle 培养基 (DMEM) 中培养。
胎牛血清(FBS)由于其高营养成分,是用于补充细胞培养基的最常见的血清。尽管其在蛋白中含量相对较低,但FBS可有效促进和维持脊椎动物、哺乳动物和昆虫细胞的生长

该产品用于进一步的制造用途。本产品不用于人类或治疗用途。

Analysis Note

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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分析证书(COA)

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Journal of virology, 76(2), 791-801 (2001-12-26)
The serotypes of adeno-associated virus (AAV) have the potential to become important resources for clinical gene therapy. In an effort to compare the role of serotype-specific virion shells on vector transduction, we cloned each of the serotype capsid coding domains
Julie L Webb et al.
The Journal of biological chemistry, 278(27), 25009-25013 (2003-04-30)
Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the formation of aggregates (Lewy bodies) in neurons. alpha-Synuclein is the major protein in Lewy bodies and rare mutations in alpha-synuclein cause early-onset PD.
Tadepally Lakshmikanth et al.
Cell reports, 32(3), 107923-107923 (2020-07-23)
The human immune system varies extensively between individuals, but variation within individuals over time has not been well characterized. Systems-level analyses allow for simultaneous quantification of many interacting immune system components and the inference of global regulatory principles. Here, we
D B Bregman et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(21), 11586-11590 (1996-10-15)
Damage to actively transcribed DNA is preferentially repaired by the transcription-coupled repair (TCR) system. TCR requires RNA polymerase II (Pol II), but the mechanism by which repair enzymes preferentially recognize and repair DNA lesions on Pol II-transcribed genes is incompletely
R J Mark et al.
Journal of neurochemistry, 68(1), 255-264 (1997-01-01)
Peroxidation of membrane lipids results in release of the aldehyde 4-hydroxynonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. Because accumulating data indicate that free radicals mediate injury and death of

实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

相关内容

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

全球贸易项目编号

货号GTIN
12103C-1L04065266960594
12103C-500ML04061838251954
12103C-1000ML04061838261434
12103C-100ML04061838717252

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