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12306C

胎牛血清

Name: 胎牛血清
Brand: SIGMA

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

别名:

FBS, FCS, 血清

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关于此项目

UNSPSC Code:

12352207

NACRES:

NA.71

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

non-USA origin

Sterility:

sterile-filtered

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Premier

quality

from USDA approved countries

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Analysis Note

内毒素和血红蛋白测试

Tested for the presence of bacteria, virus, and mycoplasma
Triple filtered with 0.1 micron membrane under aseptic conditions

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture

Vosough M, et al.

Stem Cells and Development, 22(20), 2693-2705 (2013)

Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells

Asgari S, et al.

Stem Cell Reviews and Reports, 9(4), 493-504 (2013)

Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates.

Xiao-Min Liu et al.

Nature chemical biology, 15(9), 865-871 (2019-08-07)

RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A

UDP-glucose 6-dehydrogenase knockout impairs migration and decreases in vivo metastatic ability of breast cancer cells.

Shao Thing Teoh et al.

Cancer letters, 492, 21-30 (2020-08-10)

Dysregulated metabolism is a hallmark of cancer that supports tumor growth and metastasis. One understudied aspect of cancer metabolism is altered nucleotide sugar biosynthesis, which drives aberrant cell surface glycosylation known to support various aspects of cancer cell behavior including

AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells.

Michael P Collins et al.

The Journal of biological chemistry, 295(28), 9433-9444 (2020-05-16)

The vacuolar H+-ATPase (V-ATPase) is an ATP-dependent proton pump that is essential for cellular homeostasis. V-ATPase activity is controlled by the regulated assembly of the enzyme from its component V1 and V0 domains. We previously reported that amino acid starvation

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实验方案

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全球贸易项目编号

货号	GTIN
12306C-50ML	04061837031175
12306C-100ML	04061838722003
12306C-500ML	04061838722010

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