EHS Matrix Extract

EHS Matrix Extract is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Its major components are laminin, collagen type IV, plaminogen activator and other growth factors which occur naturally in the EHS tumor.

EHS Matrix Extract will gel rapidly at 22°C to 37°C. Thaw the EHS Matrix Extract at 4°C overnight in a refrigerator. Use pre-chilled pipettes, plates and tubes when preparing matrigel. Gelled EHS Matrix Extract may be liquefied by placing in refrigerator at 4°C overnight.

Take the appropriate culture medium for your cells from the refrigerator. Decontaminate the bottle with 70% alcohol in a sterile hood.
*Keep EHS Matrix Extract on ice throughout the preparation. Use chilled pipette tips, pipettes, culture plates/flasks and tubes for preparing EHS Matrix Extract.

• Thaw EHS Matrix Extract at 4°C overnight on ice.
• Use cooled pipette tips, mix the EHS Matrix Extract on ice to homogeneity.
• Prepare EHS Matrix Extract matrix by adding chilled culture medium to EHS Matrix Extract on ice in a 1:1 ratio.
• Coat tissue culture surface evenly.

Keep the EHS Matrix Extract to surface ratio at 1ml/10cm²
For example:
2.5 ml for each T-25 flask (1.25 ml of culture medium to 1.25 ml of EHS Matrix Extract)
210 μl for each 24-well plate well (105 μL of culture medium to 105 μL of EHS Matrix Extract)

• Incubate at 37°C for one hour.
• Rinse EHS Matrix Extract with culture medium.
• EHS Matrix Extract coated tissue culture surface is now ready for use.