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Merck
CN

14533

双苯并咪唑 H 33342 三盐酸盐

suitable for fluorescence, ≥97.0% (HPLC)

别名:

2′-(4-乙氧基苯基)-5-(4-甲基-1-哌嗪基)- 2,5′-双-1H-苯并咪唑 三盐酸盐, HOE 33342, Hoechst 33342, 双苯并咪唑

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关于此项目

经验公式（希尔记法）:
C27H28N6O · 3HCl · xH2O
化学文摘社编号:
875756-97-1
分子量:
561.93 (anhydrous basis)
UNSPSC Code:
12161505
NACRES:
NA.25
PubChem Substance ID:
57647173
EC Number:
245-691-1
Beilstein/REAXYS Number:
1234011
MDL number:
MFCD00012678

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产品名称

双苯并咪唑 H 33342 三盐酸盐, suitable for fluorescence, ≥97.0% (HPLC)

InChI key

FYEVKHPLBHLWHK-UHFFFAOYSA-N

InChI

1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H

SMILES string

Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6

assay

≥97.0% (HPLC)

fluorescence

λex 340 nm; λem 510 nm
λex 355 nm; λem 465 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

200

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Application

bisBenzimide H 33342 三盐酸盐可用于研究生物化学和试剂、荧光探针、标记、颗粒和污渍、发光化合物和检测和波长指数。bisBenzimide H 33342 三盐酸盐，Hoechst 33342，已用于研究无机和有机砷化合物对人类 T 淋巴母细胞白血病细胞的影响。Hoechst 33342 也被用于研究 9 种钙拮抗剂在 HeLa 和 SN-38 抗性 HeLa（HeLa/SN100）细胞（过量表达 ABCG2/BCRP）中，对 ABCG2/BCRP 介导的抗性和转运的影响。
适用于DNA，染色体和细胞核染色。可用于荧光显微镜或流式细胞术。
最大激发波=346nm
最大发射波长=460nm

Biochem/physiol Actions

膜透性，具有低细胞毒性荧光DNA染料，插入在DNA的A-T区域。

Other Notes

适用于各种细胞类型的重要 DNA染色；作为哺乳动物细胞膜通透性的探针

Packaging

无底玻璃瓶。内含物装在插入的融合锥内。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN0628
BCCL3465
BCCJ9222
BCCH0905
BCCG8558

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the solubility of Product 14533, bisBenzimide H 33342 trihydrochloride?

    It is soluble in water at 50 mg/mL.  It will precipitate from phosphate buffer solutions

  4. When Product 14533, bisBenzimide H 33342 trihydrochloride, is dissolved in water, what is the pH?

    The pH of a 2% solution in water is approximately 1.9.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

W M Grogan et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(6), 738-746 (1981-06-01)
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells
M J Lydon et al.
Journal of cellular physiology, 102(2), 175-181 (1980-02-01)
A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is
M E Lalande et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(1), 363-367 (1981-01-01)
Flow cytometric analysis of the uptake of the DNA-specific and fluorescent probe Hoechst 33342 (HO342) offers a simple and rapid method for measuring membrane transport rates in mammalian cells and identifying cellular subpopulations that differ in their membrane transport rates.
Steven S Welc et al.
Nature communications, 10(1), 2788-2788 (2019-06-28)
Many potentially therapeutic molecules have been identified for treating Duchenne muscular dystrophy. However, targeting those molecules only to sites of active pathology is an obstacle to their clinical use. Because dystrophic muscles become extensively inflamed, we tested whether expressing a therapeutic
Ganna Aleshcheva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(6), 2303-2314 (2015-02-15)
Real and simulated microgravity induce a variety of changes in human cells. Most importantly, changes in the cytoskeleton have been noted, and studies on microtubules have shown that they are gravisensitive. This study focuses on the effects of short-term real
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